UNAIR NEWS – Five Biomedical Engineering students of Faculty of Science and聽ersitas聽Airlangga, Evlyn Anggraini Santoso, Fulky A’yunni, Fathania Nabilla, Novita Putri Rahayu, and Adita Wardani Rahmania have made an innovation through their research about聽dialyzer聽membranes (membranes for dialysis / Hemodialysis) with antibacterial properties.
Hemodialysis聽therapy聽(HD) is the most selected treatment for patients with chronic renal failure.听Based on a survey of various centers of聽hemodialysis聽, in Indonesia there are 30.7% of patients per million inhabitants who require聽hemodialysis.听Routine vascular accessed during every HD procedure can lead to bacteriological conditions and infections that can degrade the patient’s quality of life and bring death.听Besides vascular access, bacterial infections can result from dialysis fluid, reused dialyzer, water treatment at dialysis centers and dialysis machines.
The聽dialyzer聽membranes聽used so far have no antibacterial properties when there are many external factors from the process of HD treatment which can cause infection, so the albumin decreases.听The percentage of infection occurring within a month by 15%, 1-12 months 44%, and above 1 year 41%.听The occurrence of this infection is caused by gram-positive pathogens of 97% of cases, mainly by聽Staphylococcus aureus聽(60%) and聽Staphylococcus epidermis听(22%).
Therefore, under the guidance of Dr.听Prihartini Widiyanti, drg., M.Kes., The student is trying to make a聽dialyzer听尘别尘产谤补苍别蝉听蹿辞谤听hemodialysis聽therapy聽that has antibacterial properties, so as to improve the quality of life of patients with kidney failure.
“We tried to make a membranes聽dialyzer聽made of聽polyurethane听,听citric acid听补苍诲听polyethersulfon聽to later added chitosan to elicit its antibacterial properties.听Chitosan is a natural ingredient known to have high antibacterial properties, is easy to find, and the price is not too expensive, “said Evlyn Anggraini Santoso, group leader.
惭别尘产谤补苍别蝉听dialyzer聽which has been printed in two forms聽flat聽(flat) and聽hollow fiber聽is then dipped in a solution of chitosan and stored for 24 hours at 4 掳 C.听After it is washed using PBS solution and sterilization is done with soaked in glutaraldehyde for 30 minutes.
Research is packaged in the title “biocomposite Innovative Based Citric Acid-polyurethane-Chitosan as a candidate Membraness聽Dialyzer聽Antibacterials” managed to attract聽聽Higher Education (Higher Education) Kemenristek Higher Education, which then gives the fund the development of research through the Student Creativity Program Research Eksakta (PKM-PE) .
The results of this study have been tested with antibacterial tests using bacteria聽Staphylococcus aureus聽and showed a clear zone around the disc diameter of about 6.946 to 11.086 mm.听This indicates that the sample of the聽dialyzer聽membranes聽has antibacterial properties.听The antibacterial activity of chitosan derived from amine functional group that can form a bond with the cell walls of bacteria and bacteria lysis.
FTIR test results indicate the discovery of the functional groups of chitosan NH / OH (amine and hydroxyl) which constituent chitosan.听The results of measurements have been carried out using a membranes pore SEM test and obtained 0.944 渭m pore size, these results are in accordance with the pore size of the membranes filtration.
In addition,聽hemolysis聽tests have been done聽to determine the material properties when contacted directly with blood.听If the percentage of聽hemolysis is聽below 5% then the sample is considered safe for contact with blood.听From the results of the test the five samples showed percentage of聽hemolysis聽less than 5% so safe to use for聽hemodialysis聽process聽.听(*)
Editor: Bambang Bes
